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rnascope 2.5 hd duplex reagent kit  (Advanced Cell Diagnostics Inc)


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    Structured Review

    Advanced Cell Diagnostics Inc rnascope 2.5 hd duplex reagent kit
    Rnascope 2.5 Hd Duplex Reagent Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+2%2E5+hd+duplex+reagent+kit/rnascope+multiplex+fluorescent+reagent+kit+v2/pmc12209767-398-11-17
    Average 90 stars, based on 1 article reviews
    rnascope 2.5 hd duplex reagent kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Situ Hybridization:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    In Situ:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    RNAscope:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Hybridization:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Formalin-fixed Paraffin-Embedded:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Negative Control:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Control:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Variant Assay:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Cell Migration Assay:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Migration:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Migration Assay:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Incubation:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Plasmid Preparation:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Recombinant:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Virus:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).

    Multiplex Assay:

    Article Title: CXCL9 and CXCL13 shape endometrial cancer immune-activated microenvironment via tertiary lymphoid structure formation.
    Article Snippet: The manual RNAscope assay was performed using the RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics) according to the manufacturer's instructions.

    Article Title: B-cell Receptor Silencing Reveals the Origin and Dependencies of High-Grade B-cell Lymphomas with MYC and BCL2 Rearrangements
    Article Snippet: Human RAG1 and RAG2 probes (Advanced Cell Diagnostics) were hybridized using RNAscope 2.5 HD Duplex Reagent Kit (Advanced Cell Diagnostics), adopting an extended 1-hour incubation in Amplifier reagent 5 and 30-minute incubation in Amplifier reagent 6 for RAG1/RAG2 hybridization.

    Article Title: Dysregulation of lung epithelial cell homeostasis and immunity contributes to Middle East respiratory syndrome coronavirus disease severity
    Article Snippet: Reagents and kits from ACD Bio were as follows: RNAScope 2.5 HD Duplex Reagent Kit (Cat#322430), RNAScope Multiplex Fluorescent Reagent Kit v2 (Cat#323100), RNAScope probe V-MERS-CoV-O1 (channel 1) (Cat#485081), RNAScope probe V-MERS-CoV-S (channel 3) (Cat#860551-C3), RNAScope probe Ager (channel 1) (Cat#550791), RNAScope probe Sftpc (channel 2) (Cat#314101-C2), RNAScope probe Foxj1 (channel 2) (Cat#317091-C2), and RNAScope probe Scgb1a1 (channel 3) (Cat#420351-C3).



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